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Study On The Acute Toxicity Effect Of Ethanoic Extract of Dioscorea villosa Tuber On Wistar Albino Rats In Ifitedunu, Anambra State.

Abstract

Lorke’s method was used to study the acute toxicity effect of ethanoic extract from the Dioscorea villosa tuber on Wistar albino rats. The study involved intra-peritoneal administration of different doses of the extract to the groups of the male albino rats. Signs accompanying toxicity and possible death of animals were monitored for two weeks to ascertain the lethal dose (LD₅₀) of the extract.The animals weight was checked at every three days intervals. At the end of the two weeks study, all the animal in all the dose groups were sacrificed. The safety usage of the ethanoic extracts from the Dioscorea villosa tuber is emphasized. There were no possible changes in behaviors and no mortality after 24hours observation in two phases. The LD50, being greater than 5000 mg/kg b.w., is thought to be safe as suggested by Lorke (1983).

Keywords: Acute toxicity, Dioscorea villosa, albino rats.