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Isolation, Screening and Identification Of Multi – Metal Resistant Fungi Isolated From Biogas Slurry Sample

ABSTRACT

The potentials of filamentous fungi as bioaccumulating agents of heavy metal toxicants in soil or aquatic ecosystems have been widely documented as better and efficient option for bioremediation technique. This study was undertaken to isolate, screen and identify multi – metal resistant fungi isolated from biogas slurry samples. Water samples were collected, fungi strains isolated, screened for multi - resistant abilities and finally identified using standard microbiological and molecular procedures, respectively. The results revealed that there were increase in the growth diameters of the fungal strains as the concentrations (10 - 200 pm) of all heavy metals from day 0 to day 20. Some of the metals were inhibitory while some were stimulatory effects at certain levels to the fungal biomass. The growth of the fungi at increasing concentrations revealed that these strains especially strains S6 and S9 possess multi – resistant genes and thereby making adaptable to these inorganic pollutants. The strains S6 and S9 were later identified as Aspergillus niger and Colletotrichum sp., respectively and their heavy metal bioremediation applicabilities to heavy metal contaminated soil or aquatic environment are therefore recommended.

Key words: Aspergillus niger, Bioaccumulation, Colletotrichum sp., Heavy metal, Multi - Resistant