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Supersizing The Inhibitory Activity Of Xylopia Aethiopica Extract Against Vibrio Cholerae Using Doxycycline

ABSTRACT

Studies have shown that multi-dung resistant Vibrio species is a well-recognized and documented problem worldwide especially in the developing countries, and the gene associated with this resistance are encoded in the plasmid. This study was carried out to evaluate the effect of Xylopia aethiopica extract on different strains of Vibrio species. Samples were drawn from streams and screened for the presence of test isolates using standard microbiological techniques. The phytochemical constituents of Xylopia aethiopica (XA) was quantitatively determined using gravimetric and spectrophotometric methods. The antibacterial activity was carried out using agar-welled diffusion method. Vibrio cholerae 01 bio var EI Tor strain C6709 (VCC6), Vibrio cholerae 01 bio var EI Tor strain P27459 (VCP2) and Vibrio cholerae 01 bio var EI Tor E7946(VCE7) were detected in the studied sample of which VCC6 was significantly (p < 0.05) most predominant in the stream samples. The study also showed that XA contain alkaloids, steroids, cardiac glycosides, phenolics, flavonoids, tannins, saponins and was able to significantly (p < 0.05) inhibit the strains of Vibrio species, of which the inhibition was significantly (p < 0.05) increased when combined with Doxycycline (DX), and inhibited VCE7 most. From the above study, VCC6, VCE7 and VCP2 were seen in the studied samples, and the extracts showed pronounced activity the isolates, of which the activity was boosted when combined with DX.

Key words: Vibrio cholerae, Xylopia aethiopica, phytochemical, Doxycycline