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INVESTIGATION ON THE OCCURRENCE, SALINITY TOLERANCE, AND NON - VIRULENCE CHARACTERISTICS OF BACTERIAL STRAINS OBTAINED FROM WATER AND SEDIMENT SAMPLES OF THE MAYAMI SALINE ENVIRONMENT

Published in Volume 4, Issue 1

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Abstract

Halotolerant bacteria are increasingly recognized for their potential in biotechnological
applications, particularly in saline wastewater treatment. This study investigated the occurrence, salinity tolerance, and virulence characteristics of bacterial isolates obtained from salty water and
sediment samples of the Mayami saline environment. Standard plate count techniques revealed
high bacterial loads ranging from 1.80 × 10⁶ to 3.30 × 10⁶ CFU/g, indicating that the
environment supports abundant halotolerant microbial populations. Salinity tolerance assays
showed variable adaptability among isolates, with strain MS5 demonstrating the highest
tolerance (2 – 10 % NaCl), while others exhibited limited growth at elevated salinity. Virulence
screening using blood agar, Indian ink, and Coomassie brilliant blue assays confirmed that
selected isolates (MS4 and MS5) were non-virulent, apathogenic, and non-haemolytic. These
findings suggest that the isolates, particularly MS5, are promising candidates for safe application
in saline wastewater bioremediation. The study highlights the ecological and biotechnological
relevance of halotolerant bacteria in hypersaline environments.

Keywords

ApathogenicBiotechnological relevancenon-haemolyticnon-virulentSalinity tolerance

How to Cite This Paper

Okoye, C. P., Mbachu, I. A. C., Uba, B. O. (2026). INVESTIGATION ON THE OCCURRENCE, SALINITY TOLERANCE, AND NON - VIRULENCE CHARACTERISTICS OF BACTERIAL STRAINS OBTAINED FROM WATER AND SEDIMENT SAMPLES OF THE MAYAMI SALINE ENVIRONMENT. Tropical Journal of Applied Natural Sciences, Volume 4, Issue 1, 1-26.
Okoye, C. P., Mbachu, I. A. C., Uba, B. O.. "INVESTIGATION ON THE OCCURRENCE, SALINITY TOLERANCE, AND NON - VIRULENCE CHARACTERISTICS OF BACTERIAL STRAINS OBTAINED FROM WATER AND SEDIMENT SAMPLES OF THE MAYAMI SALINE ENVIRONMENT." Tropical Journal of Applied Natural Sciences, vol. Volume 4, Issue 1, 2026, pp. 1-26.
Okoye, C. P., Mbachu, I. A. C., Uba, B. O.. "INVESTIGATION ON THE OCCURRENCE, SALINITY TOLERANCE, AND NON - VIRULENCE CHARACTERISTICS OF BACTERIAL STRAINS OBTAINED FROM WATER AND SEDIMENT SAMPLES OF THE MAYAMI SALINE ENVIRONMENT." Tropical Journal of Applied Natural Sciences Volume 4, Issue 1 (2026): 1-26.
@article{investigationontheoccurrence,salinitytolerance,andnon-virulencecharacteristicsofbacterialstrainsobtainedfromwaterandsedimentsamplesofthemayamisalineenvironment2026,
  author = Okoye, C. P., Mbachu, I. A. C., Uba, B. O.,
  title = INVESTIGATION ON THE OCCURRENCE, SALINITY TOLERANCE, AND NON - VIRULENCE CHARACTERISTICS OF BACTERIAL STRAINS OBTAINED FROM WATER AND SEDIMENT SAMPLES OF THE MAYAMI SALINE ENVIRONMENT,
  journal = Tropical Journal of Applied Natural Sciences,
  year = 2026,
  volume = Volume 4, Issue 1,
  pages = 1-26
}
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