Published in Volume 4, Issue 1
Diabetic patients are typically associated with gut dysbiosis. However, little or no research has
been conducted on the restoration of gut microbiota in diabetic patients in Nigeria, particularly
Anambra State. This study focused on the amelioration of gut stability in diabetic patients
using Nauclea latifolia fruit extracts (NLFE). Forty stool samples were collected (20 from
diabetic patients and 20 from non-diabetic subjects). Comprehensive digestive stool analysis
(CDSA) was performed using standard plate counts and instrumental techniques. Unique
bacterial isolates from diabetic patients were characterized. An in vivo human trial assessed the
effect of NLFE on gut stability. Data were analyzed using one-way ANOVA and Student's t-test
at 95% confidence level. CDSA revealed decreased counts
of Clostridium (CC), Bacteroides (BAC), and Lactobacillus (LC), with a significant decrease (p
< 0.05) in Bifidobacterium counts (BC) among diabetic patients. Following NLFE administration, CC, BAC, BC, and LC showed progressive restoration, reaching significance (p < 0.05) after six
months. NLFE demonstrated pronounced activity, restoring 80% of gut stability in diabetic
patients. This study provides the first evidence in Anambra State, Nigeria, that Nauclea
latifolia fruit extract effectively restores gut microbiota in diabetic patients, offering a potential
dietary intervention for diabetes-associated dysbiosis.
Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M. (2026). AMELIORATION OF GUT STABILITY OF DIABETIC PATIENTS USING NAUCLEA LATIFOLIA FRUIT EXTRACTS. Tropical Journal of Applied Natural Sciences, Volume 4, Issue 1, 27-38.
Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M.. "AMELIORATION OF GUT STABILITY OF DIABETIC PATIENTS USING NAUCLEA LATIFOLIA FRUIT EXTRACTS." Tropical Journal of Applied Natural Sciences, vol. Volume 4, Issue 1, 2026, pp. 27-38.
Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M.. "AMELIORATION OF GUT STABILITY OF DIABETIC PATIENTS USING NAUCLEA LATIFOLIA FRUIT EXTRACTS." Tropical Journal of Applied Natural Sciences Volume 4, Issue 1 (2026): 27-38.
@article{ameliorationofgutstabilityofdiabeticpatientsusingnauclealatifoliafruitextracts2026,
author = Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M.,
title = AMELIORATION OF GUT STABILITY OF DIABETIC PATIENTS USING NAUCLEA LATIFOLIA FRUIT EXTRACTS,
journal = Tropical Journal of Applied Natural Sciences,
year = 2026,
volume = Volume 4, Issue 1,
pages = 27-38
}
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