Published in Volume 4, Issue 1
Studies have shown that diabetic patients are usually associated with dysbiosis. Several studies
have been done on restoration ofblood glucose level but little or no study has been done on pH
and short chain fatty acids level of diabetic patients in Nigeria majorly Anambra State. Hence
this study focused on Assessment ofthe pH, short chain fatty acids and blood Glucose level of
type 2 diabetic patients administered with Nauclea latifolia fruit extracts. A total of 40 stool
samples; 20 samples from diabetic (10 males and 10 females) patients and 20 samples from non- diabetic (10 males and 10 females) subjects, were collected and analyzed for pH, short chain
fatty acids (SCFA) / (butyrate and propionate) and blood glucose level using instrumentational
techniques. The unique bacterial isolates encountered in the gut of diabetic patients were
appropriately characterized. Phytochemical constituents ofNauclea latifolia fruit extracts (NLFE)
was quantitatively determined using gravimetric and spectrophotometric methods; In vivo
technique using human subjects was employed to determine the effect of NLFE on pH, short
chain fatty acid and blood glucose level. The data generated were analyzed using one-way
Analysis of Variance (ANOVA) at 95% confidence level, and compared using student “t” test. There were decreased in stool pH and blood glucose, and significant (P<0.05) decreased in SCFA
among the diabetic patients. Alkaloids, saponins, phenolics, tannins, flavonoids, steroids and
glycosides were detected from NLFE. The study has revealed that NLFE showed pronounce
activity and was able to restore 80% of pH, short chain fatty acids and blood glucose level of
diabetic patients.
Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M., Chigbu, C. C. (2026). ASSESSMENT OF THE pH, SHORT CHAIN FATTY ACIDS AND BLOOD GLUCOSE LEVEL OF TYPE 2 DIABETIC PATIENTS ADMINISTERED WITH NAUCLEA LATIFOLIA FRUIT EXTRACTS. Tropical Journal of Applied Natural Sciences, Volume 4, Issue 1, 39-51.
Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M., Chigbu, C. C.. "ASSESSMENT OF THE pH, SHORT CHAIN FATTY ACIDS AND BLOOD GLUCOSE LEVEL OF TYPE 2 DIABETIC PATIENTS ADMINISTERED WITH NAUCLEA LATIFOLIA FRUIT EXTRACTS." Tropical Journal of Applied Natural Sciences, vol. Volume 4, Issue 1, 2026, pp. 39-51.
Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M., Chigbu, C. C.. "ASSESSMENT OF THE pH, SHORT CHAIN FATTY ACIDS AND BLOOD GLUCOSE LEVEL OF TYPE 2 DIABETIC PATIENTS ADMINISTERED WITH NAUCLEA LATIFOLIA FRUIT EXTRACTS." Tropical Journal of Applied Natural Sciences Volume 4, Issue 1 (2026): 39-51.
@article{assessmentoftheph,shortchainfattyacidsandbloodglucoseleveloftype2diabeticpatientsadministeredwithnauclealatifoliafruitextracts2026,
author = Orji, C. C., Orji, O. B., Iheukwumere, I. H., Enweani, I. B., Umeaku, C. N., Orji, S. I., Nagogo, H. S., Yakubu, I. M., Chigbu, C. C.,
title = ASSESSMENT OF THE pH, SHORT CHAIN FATTY ACIDS AND BLOOD GLUCOSE LEVEL OF TYPE 2 DIABETIC PATIENTS ADMINISTERED WITH NAUCLEA LATIFOLIA FRUIT EXTRACTS,
journal = Tropical Journal of Applied Natural Sciences,
year = 2026,
volume = Volume 4, Issue 1,
pages = 39-51
}
Download BibTeX